ArcDia TRF Metalloporphyrins in time-resolved fluorometry and multicolor microscopy

ArcDia has developed new labelling reagents and related time-resolved fluorescence instrument technology for use in bioaffinity assays. These ArcDia TRF -labelling reagents are based on monofunctional metalloporphyrins. These labelling reagents have a long phosphoresence decay time and they provide very high detection sensitivity with time-resolved fluorometry. ArcDia TRF -metalloporphyrins can be used as labelling reagents in separation assays, homogenous assays or as labeling reagents in automated multicolor microscopy.

ArcDia TRF -reagent family consist of two monofunctional metalloporphyrins: monofunctional Pt-porphyrin and monofunctional Pd-porphyrin. The fluorescence (phosphorescence) decay times of these labelling reagents are 100 microseconds and 1000 microseconds, respectively. These labelling reagents have been produced with several different functional linkers that allow easy conjugation with biomolecules.

Licences for ArcDia TRF -labelling reagents is offered to domestic and foreign biotechnology and in-vitro diagnostics industry. Patents pending.

Multicolor microscope labelling and imaging has been done in collaboration with Lahja Seveus, PhD, Pharmacia Biotech and Ari Kuusisto, PerkinElmer LifeSciences, Wallac

Development of ArcDia TRF -labelling reagents has been supported by National Technology Agency Tekes.