TPX technology for infectious disease diagnostics
mariPOC® test system applies patented ArcDia™ TPX technique for infectious disease diagnostics. This technique is based on two-photon excitation of fluorescence detection, which allows separation-free analysis from microvolumes. The technique applies polystyrene microparticles as solid phase reaction carries for the immunocomplex formation. As a result of the antibody-antigen interactions, three-component immunocomplexes of MAb-Ag-MAb* (monoclonal antibody – antigen – labelled monoclonal antibody) are formed on the microspheres in proportion to the analyte concentration.
The detection of the immunoassay fluorescence signal is carried out by means of two-photon excitation (λex = 1064 nm) from the surface of individual microspheres. Microspheres are trapped into the laser focus by optical forces, and the bound and the unbound tracer fractions are separated optically. Both the microsphere signal (specific signal) and the solution background signal (unbound tracer and matrix) are recorded. This allows for efficient compensation of matrix effects, and eliminates the need of calibration of the fluorescence detector.
- Separation-free fluorescence measurement
- Single-step assay
- Real-time monitoring of reaction kinetics
- Performance and accuracy are independent of reaction volume
- Small reaction volumes
- Compatible with dried reagents
- Excellent sensitivity
- Wide dynamic range
- Excellent precision
ArcDia™ TPX technology development has been supported by the Academy of Finland, Tekes
(The Finnish Funding Agency for Technology and Innovation) and European Union.