mariPOC® and ArcDia™ TPX technology
mariPOC® utilizes the ArcDia™ TPX (Two-Photon Excitation) assay technique for highly sensitive and extremely specific direct pathogen detection. The technique is widely described in scientific publications. It is a proprietary technology of the Finnish Company ArcDia International Ltd., the developer and manufacturer of mariPOC®.
High sensitivity and specificity
mariPOC® provides the best antigen detection sensitivity at the point-of-care level. Antigen detection takes advantage of the natural in vivo multiplication of pathogens in the human body which leads to detectable antigen levels in acute infections. It does not return clinically false positive results as the ex vivo amplification of nucleic acids applied in PCR. The superior specificity is the unique feature of the mariPOC® technique. It results from the use of the sandwich immunoassay principle, polystyrene microparticle solid phase and the TPX detection technology.
mariPOC® is unique
Direct detection of pathogen antigens takes place on polystyrene microparticles as the solid reaction phase. Laser-excited (λex=1064 nm) fluorescence of individual microparticles (specific fluorescence) is measured, separation-free, one at a time. The fluorescence brightness of the particles is in direct proportion to target concentration in the sample. Also, the fluorescence signal of the surrounding solution (unbound tracer and sample matrix) is recorded. This enables efficient elimination of matrix interferences and autoverification of sample analysis.
Finally a sophisticated data reduction algorithm returns the quantitative test result. Thanks to the TPX technology, the products are extremely homogeneous and specific. TPX technology is the new standard in rapid direct pathogen detection. The unique technology is easy to use, efficient and accurate. mariPOC® detection is automated. The process can be applied even to complex sample materials such as stools and aspirates.
The reagents are stored dried in test reaction wells. Typically multianalyte testing is realized by having parallel reaction wells, one for each target pathogen.